FastQCFastQC Report
Tue 20 Jun 2017
I894_S90.scythe.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameI894_S90.scythe.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15645659
Sequences flagged as poor quality0
Sequence length1-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1330030.8500952244964561No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT437040.27933626829013725No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267390.17090363531507366No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239510.15308399601448555No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210830.13475303277413883No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195980.12526158214236932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCC323450.037.4966431
CCCCCCT167950.031.801213
CCCCCTT193900.027.4013564
TATGCCG29050.019.46296348-49
CCCCTTT275900.019.3748025
CGTATGC31950.017.3280646-47
CCCTTTT310750.017.3155886
GTCCGCT22550.016.1600591
ACCCGCT34450.015.97142494
ATGCCGT36050.015.941915548-49
GTGCGCG5359.094947E-1214.8453371
GAGCACA80500.014.83464059
CCTTTTT371950.014.5789457
CCCGTGT58650.014.3383561
CCCGCTT29700.014.0000131
CCGACAT53950.013.2493591
GTATGCC44550.013.00156146-47
GTCCGTT36500.012.6717681
TCGTATG46750.012.2380644-45
TCGGAAG95350.012.23648553
Produced by FastQC (version 0.11.2)