FastQCFastQC Report
Tue 20 Jun 2017
I894_S90.scythe.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameI894_S90.scythe.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15645659
Sequences flagged as poor quality0
Sequence length1-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT459830.29390260902401105No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA239810.15327574249189505No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187250.11968175964975332No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179540.11475387518032956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCT199500.027.74265
CCCCCTT211650.027.180926
CCCCTTT218750.026.9259937
CCCTTTT268650.022.0294938
GAGCGTC53550.021.3095789
CCTTTTT296750.020.049989
CGTGTAG33100.016.66244314-15
AGAGCGT72450.016.4670648
CCCCCCC882300.016.3453031
TGTAGGG35150.016.0249316-17
GTAGGGA38750.014.89680118-19
AGTAGTC46500.014.0405497
GCCAGTA47650.013.9540683
TCGTGTA41200.013.44305814-15
AAGAGCG88200.012.8342567
CCAGTAG51250.012.8065885
GCCGTAT45550.012.80033246-47
GTAGTCA53700.012.6827848
AGTGTAG46100.012.5276428-29
CTGCCAG52650.012.5069881
Produced by FastQC (version 0.11.2)