FastQCFastQC Report
Tue 20 Jun 2017
I894_S90.sickle.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameI894_S90.sickle.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15357880
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1229360.8004750655689458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCC190600.036.3682141
CCCCCCT100050.031.1076623
CCCCCTT121800.025.6664584
TATGCCG29850.018.7688148-49
CGTATGC31500.017.55234346-47
GTCCGCT20300.017.5169661
CCCCTTT197900.015.9366875
ATGCCGT37250.015.66431948-49
CCGACAT53250.015.5237841
ACCCGCT32800.015.46197594
GAGCACA81200.014.9526029
CCCTTTT226750.013.9870626
CCCGTGT58300.013.7830351
CGCGTAT26150.013.5982551
TCGGAAG90050.013.0249063
GTATGCC44100.013.01149146-47
CGGGGCG18850.012.9376976-77
ACGTCTG45800.012.90234514-15
CCCAGGT42000.012.6448231
TCGTATG47350.012.45380844-45
Produced by FastQC (version 0.11.2)