FastQCFastQC Report
Tue 20 Jun 2017
I894_S90.sickle.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameI894_S90.sickle.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15357880
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT411580.2679927177448971No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA233850.15226710978338157No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158370.10311970141712266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCT177350.029.7257655
CCCCCTT202950.027.1828966
CCCCTTT210800.026.6566287
CCCTTTT254200.022.4160068
GAGCGTC54050.021.491499
CCTTTTT279100.020.4120529
TCCCCCC28900.019.8092291
CCCCCCC699850.019.6781331
AGAGCGT68150.017.3200868
CGTGTAG32750.017.1625514-15
TGTAGGG34400.016.55446816-17
GTAGGGA38150.015.2399318-19
AGTAGTC45650.014.3964736
GCCAGTA43650.014.3869353
GCCGTAT43050.013.46777446-47
CTGCCAG49900.013.3083081
TCGTGTA43400.013.10958614-15
CCAGTAG48150.013.0462324
AAGAGCG89250.012.8099047
AGTGTAG46950.012.73993328-29
Produced by FastQC (version 0.11.2)