FastQCFastQC Report
Tue 20 Jun 2017
I894_S90.singles.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameI894_S90.singles.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283435
Sequences flagged as poor quality0
Sequence length20-100
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75702.670806357718701No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45341.5996612980048335No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41961.4804099705399827No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31811.1223031735671318No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27370.9656535008026532No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21640.7634907474376842No Hit
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18890.666466738405631No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16620.5863778291318997No Hit
CCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13230.4667736870887505No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8170.28824951046977265No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5820.2053380845696544No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5730.20216275336496903No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5400.19051987228112266No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.18875579938963077No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5050.17817136204067952No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.15347434155979325No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.14042020216275336No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3970.140067387584455No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.12807169192231022No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.12313228782613297No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3440.12136821493464109No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.12066258577804435No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3410.12030977119974597No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.11960414204314923No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3350.11819288372995572No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3340.11784006915165735No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3260.11501755252527034No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3180.11219503589888336No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.11007814842909308No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3080.10866689011589958No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3070.10831407553760122No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.10796126095930283No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.10796126095930283No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2950.1040803005980207No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.1026690422848272No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2860.10090496939333535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCC107750.037.2200471
CCCCCCT59450.031.0425533
CCCCCTT64750.028.626354
CCCCTCC1004.5148354E-628.3281751
CCCTCCC1004.5148354E-628.3281752
CCCCTTT70150.026.6530175
CCCTTTT73250.025.2766846
CCTTTTT75750.024.4424727
CTTTTTT92200.020.2988648
CACGCTT1050.00554730919.2708681
CTCCCCT1350.001129193617.9477734
CTCGGAT1500.002253444216.1875291
CCTCCCC2107.686642E-515.4166951
TCGGATT1650.004252117614.7159362
GAATCTG1700.005258416314.2526434
TCCCCTT1700.005258416314.2526435
GTGGTGA1950.00892084910.47418982-83
GTGGGGA2700.00945912910.3901077
GGGGGGG5858.429927E-58.2835873
Produced by FastQC (version 0.11.2)