FastQCFastQC Report
Tue 20 Jun 2017
I894_S90_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameI894_S90_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15645659
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1330050.8501080075949501No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT437180.279425749979595No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267420.17092280996281461No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239570.15312234530996746No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210890.1347913820696207No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196110.12534467228258012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCC323600.037.918611
CCCCCCT168000.032.152063
CCCCCTT194000.027.6976264
CCCCTTT276000.019.587295
CCCTTTT310850.017.5062036
GTCCGCT22550.016.3431991
ACCCGCT34450.015.14432694
GTGCGCG5359.094947E-1215.0135751
CCTTTTT372100.014.7508637
TATGCCG39350.014.51027248-49
CCCGTGT58650.014.5008511
CCCGCTT29700.014.1586731
CCGACAT53950.013.3995121
ATGCCGT45150.012.85444148-49
GTCCGTT36550.012.7978441
CGTATGC44600.012.539103546-47
CCCCGGT15650.012.0763211
CCCGACT82300.011.9987351
CTTTTTT472000.011.8876668
CGCGTAT23200.011.6084931
Produced by FastQC (version 0.11.2)