Bismark

Bismark Processing Report

inputSRR9833662.1_R1_001.fq and inputSRR9833662.1_R2_001.fq

Data processed at 10:04:54 on 2020-12-01


Alignment Stats

Sequence pairs analysed in total 198056601
Paired-end alignments with a unique best hit 93388505
Pairs without alignments under any condition 97718489
Pairs that did not map uniquely 6949607
Genomic sequence context not extractable (edges of chromosomes) 2

Cytosine Methylation

Total C's analysed 4705912213
Methylated C's in CpG context 161781790
Methylated C's in CHG context 24566161
Methylated C's in CHH context 93040204
Methylated C's in Unknown context 1384306
Unmethylated C's in CpG context 36707495
Unmethylated C's in CHG context 1025184113
Unmethylated C's in CHH context 3364632450
Unmethylated C's in Unknown context 3632894
Percentage methylation (CpG context) 81.5%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 2.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 46730941 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 46657562 original bottom strand

Deduplication

Alignments analysed 93388503
Unique alignments 88478121
Duplicates removed 4910382
Duplicated alignments were found at 3746636 different positions

Cytosine Methylation after Extraction

Total C's analysed 3464494167
Methylated C's in CpG context 114586013
Methylated C's in CHG context 21338090
Methylated C's in CHH context 80770801
Unmethylated C's in CpG context 26536143
Unmethylated C's in CHG context 753176300
Unmethylated C's in CHH context 2468086820
Percentage methylation (CpG context) 81.2%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 3.2%

M-Bias Plot