Bismark

Bismark Processing Report

inputSRR9833662.1_R1_001.fq and inputSRR9833662.1_R2_001.fq

Data processed at 10:48:37 on 2020-11-20


Alignment Stats

Sequence pairs analysed in total 4880925
Paired-end alignments with a unique best hit 4879902
Pairs without alignments under any condition 103
Pairs that did not map uniquely 920
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 181977383
Methylated C's in CpG context 5989125
Methylated C's in CHG context 259847
Methylated C's in CHH context 899804
Methylated C's in Unknown context 573
Unmethylated C's in CpG context 1226522
Unmethylated C's in CHG context 39982053
Unmethylated C's in CHH context 133620032
Unmethylated C's in Unknown context 7029
Percentage methylation (CpG context) 83.0%
Percentage methylation (CHG context) 0.6%
Percentage methylation (CHH context) 0.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2442984 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 2436918 original bottom strand

Deduplication

Alignments analysed 4879902
Unique alignments 4685610
Duplicates removed 194292
Duplicated alignments were found at 190357 different positions

Cytosine Methylation after Extraction

Total C's analysed 141588495
Methylated C's in CpG context 4650379
Methylated C's in CHG context 202702
Methylated C's in CHH context 704669
Unmethylated C's in CpG context 948823
Unmethylated C's in CHG context 31033087
Unmethylated C's in CHH context 104048835
Percentage methylation (CpG context) 83.1%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot