Annotation via biomart

Annotation from BioMart with Ensembl names is most flexible way to retrieve tabular annotation for an organism.

1. The Biomart start page should look like …

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2. First select the dataset, for gene expression experiment select Ensembl Genes 95 (version 95).

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3. Then the Organism, Here Human genes which is based on the GRC38.p12 genome.

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4. You can choose to filter to only a subset of genes. Or a chromosome, or regions. We won’t filter here. BY default, all genes in the genome are selected.

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5. Next select the attributes you want in the table.

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6. Expand the ‘GENE’ tab.

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7. Click “Results” (Top left -ish).

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7. Select “GO”, to download a tab-separated value (tsv) file.

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8. Put the file into our working directory, rename to “ensembl_hg_95.tsv” and open the file in Excel to view the annotation.