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      mRNA-Seq Workshop

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Introduction and Lectures
Intro to the Workshop and Core
Schedule
What is Bioinformatics/Genomics?
Experimental Design and Cost Estimation
RNA Sequencing Technologies - Dr. Lutz Froenicke
Support
Using Zoom
Using Slack
Cheat Sheets
Software and Links
Scripts
Prerequisites
CLI - Logging in and Transferring Files
CLI - Intro to Command-Line
CLI - Advanced Command-Line (extra)
CLI - Running jobs on the Cluster and using modules
R - Getting Started
R - Intro to R
R - Prepare Data in R (extra)
R - Data in R (extra)
More Materials (extra)
Data Reduction
Files and Filetypes
Prepare dataset
Preprocessing raw data
Indexing a Genome
Alignment with Star
Generating counts tables
Alignment/Counts with Salmon (Extra)
Data analysis
Prepare R for data analysis
Annotation from BioMart
Differential Expression Analysis
Pathway Analysis
Comparison between STAR and Salmon
ETC
Closing thoughts
Workshop Photos
Github page
Report Errors
Biocore website

Schedule

As the workshop progresses the schedule may be updated

Monday, July 27

8:50 am Zoom session starts
9:00 am Welcome, format and expectations and introductions
9:30 am Connections, logging in, environment
9:45 am Intro CLI/Adv CLI to Command Line
10:00 am Hands on
10:15 am 15 minute break
10:30 am Intro CLI/Adv CLI to Command Line
10:45 am Hands on
11:00 am Intro CLI/Adv CLI to Command Line
11:15 am Hands on
11:30 am Intro CLI/Adv CLI to Command Line
11:45 am Hands on
12:00 pm Lunch hour
1:00 pm Genomics/Bioinformatics Talk
2:00 pm Running jobs on a cluster
2:15 pm Hands on
2:30 pm Running jobs on a cluster
2:45 pm Hands on
3:00 pm 15 minute Break
3:15 pm Files and file types (fastq)
3:30 pm Dataset description and setup
3:45 pm Hands on
4:00 pm Dataset description and setup
4:15 pm Hands on
4:45 pm Recap and Homework
5:00 pm Zoom session ends

Tuesday, July 28

8:50 am Zoom session starts
9:00 am Recap and check everyones session
9:15 am Data checkin, preprocessing
9:30 am Hands on
9:45 am preprocessing
10:00 am Hands on
10:15 am 15 minute break
10:30 am preprocessing
10:45 am Hands on
11:00 pm preprocessing
11:15 pm Hands on
11:30 pm preprocessing
11:45 pm Hands on
12:00 pm Lunch hour
1:00 pm Technology Seminar by Lutz Froenicke
2:00 pm preprocessing
2:45 pm 15 minute break
2:30 pm Hands on
3:00 pm preprocessing QA/QC
3:30 pm Hands on
4:00 pm preprocessing Recap
4:30 pm Aligments References
3:30 pm Hands on
4:45 pm Recap and Homework and Extra
5:00 pm Zoom session ends

Wednesday, July 29

8:50 am Zoom session starts
9:00 am Recap and questions
9:10 am preprocessing QA/QC
9:20 am Files and file types (fasta/gtf)
9:30 am Aligments
10:00 am Hands on
10:30 am 15 minute break
10:45 am Aligments
11:15 am Hands on
11:45 am Aligments Recap
12:00 pm Lunch hour
1:00 pm Files and file types (sam/bam)
1:30 pm Creating counts tables
2:00 pm Hands on
2:30 pm 15 minute break
2:45 pm Alignment with Salmon
3:15 pm Hands on
4:45 pm Closing
5:00 pm Zoom session ends

Thursday, July 30

8:50 am Zoom session starts
9:00 am Recap and questions
9:15 am Intro/Adv R
9:30 am Hands on
9:45 am Intro/Adv R
10:00 am Hands on
10:15 am 15 minute break
10:30 am Intro/Adv R
11:00 am Hands on
11:45 am Prepare R for DE
12:00 pm Lunch hour
1:00 pm Experimental Design
2:00 pm Retreiving Annotation
2:20 pm Hands on
2:45 pm 15 minute break
3:00 pm Differential Expression Analysis
3:20 pm Hands on
3:40 pm Differential Expression Analysis
4:00 pm Hands on
4:20 pm Differential Expression Analysis
4:30 pm Hands on
4:45 pm Recap, Homework, and Extra
5:00 pm Zoom session ends

Friday, July 31

8:50 am Zoom session starts
9:00 am Recap and questions
9:30 am Differential Expression Analysis
10:00 am Hands on
10:30 am Differential Expression Analysis
11:00 am Hands on
11:15 am Differential Expression Analysis
11:40 am Hands on
12:00 pm Lunch hour
1:00 pm Differential Expression Analysis
1:30 pm Hands on
2:00 pm Functional Enrichment Analysis
2:30 pm Hands on
3:00 pm 15 minute break
3:15 pm Functional Enrichment Analysis
3:45 pm Hands on
4:15 pm Star vs Salmon
4:45 pm Closing
5:00 pm Zoom session ends