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      RNA-Seq Analysis

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Introduction and Lectures
Intro to the Workshop and Core
Schedule
What is Bioinformatics/Genomics?
Experimental Design and Cost Estimation
RNA Sequencing Technologies - Dr. Lutz Froenicke
Support
Zoom
Slack
Cheat Sheets
Software and Links
Scripts
Prerequisites
CLI
R
Cluster Computing
Data Reduction
Files and Filetypes
Prepare dataset
Preprocessing raw data
Indexing a Genome
Alignment with Star
Generating counts tables
Alignment/Counts with Salmon (Extra)
Data analysis
Prepare R for data analysis
Annotation from BioMart
Differential Expression Analysis
DE Quizzes R Code
Pathway Analysis
Enrichment Quizzes R Code
Comparison between STAR and Salmon
ETC
Closing thoughts
Workshop Photos
Github page
Report Errors
Biocore website

Schedule

All posted times are Pacific time zone. At least one break will be provided each morning and afternoon.

Monday, June 14

8:50 am Zoom session starts
9:00 am Introduction to the workshop and the Core
9:45 am Getting set up
10:00 am Command Line Prerequisites / Review
11:00 am Break
11:10 am Files and file types
12:00 pm Lunch
1:00 pm Technology Talk by Lutz F.
3:00 pm Prepare dataset
5:00 pm Zoom session ends

Tuesday, June 15

8:50 am Zoom session starts
9:00 am Preprocessing raw data
12:00 pm Lunch
4:00 pm Indexing a Genome
5:00 pm Zoom session ends

Wednesday, June 16

8:50 am Zoom session starts
9:00 am Alignment with STAR
12:00 pm Lunch
1:00 pm Generating counts tables
3:00 pm Prepare R for Analysis
4:00 pm What is Bioinformatics?
5:00 pm Zoom session ends

Thursday, June 17

8:50 am Zoom session starts
9:00 am R Prerequisites / Review
12:00 pm Lunch
1:00 pm Experimental Design
2:00 pm Differential Expression Analysis
5:00 pm Zoom session ends

Friday, June 18

8:50 am Zoom session starts
9:00 am Differential Expression Analysis
12:00 pm Lunch
1:00 pm Enrichment
3:30 pm Comparison: STAR vs Salmon
5:00 pm Zoom session ends