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      RNA-Seq Analysis

Home
Introduction and Lectures
Intro to the Workshop and Core
Schedule
What is Bioinformatics/Genomics?
Experimental Design and Cost Estimation
RNA Sequencing Technologies - Dr. Lutz Froenicke
Support
Zoom
Slack
Cheat Sheets
Software and Links
Scripts
Prerequisites
CLI
R
Cluster Computing
Data Reduction
Files and Filetypes
Prepare dataset
Preprocessing raw data
Indexing a Genome
Alignment with Star
Generating counts tables
Alignment/Counts with Salmon (Extra)
Data analysis
Prepare R for data analysis
Annotation from BioMart
Differential Expression Analysis
DE Quizzes R Code
Pathway Analysis
Enrichment Quizzes R Code
Comparison between STAR and Salmon
ETC
Closing thoughts
Workshop Photos
Github page
Report Errors
Biocore website

Online Workshops with Zoom

Course lecture, discussions, and one-on-one help/troubleshooting will be conducted using a zoom meeting.

Because video is involved, we ask everyone to be respectful and we reserve the right to remove someone if they are being disrespectful or disruptive.

Basic Controls

conrtol menu

The basic controls can be found at the bottom of the Zoom window. These controls allow participants to control their audio and video. You will automatically be muted on joining the call, but can unmute yourself at any time. When the “Participants” icon in the control menu is selected, a panel will expand from the right side of the Zoom window to display a list of participants.

participants panel

Non-verbal Responses

During lectures, our instructors will occasionally request non-verbal feedback from all participants to quickly assess how well the group is following the material, gauge interest in a topic, and keep track of the number of participants that have completed a hands-on exercise. These options can be found at the bottom of the participants panel.

nonberbal response options

Asking Questions

In addition to non-verbal feedback, we want to hear from participants during lecture sections. Please raise your hand using the “raise hand” option under “more” beneath the participants panel to get the speaker’s attention. The instructor will invite you to ask your question, and you should unmute yourself and speak. When your question has been answered, lower your hand.

Breakout rooms

During hands-on sections of the course, breakout rooms may be opened to allow many participants to receive one-on-one assistance at the same time. When breakout rooms are opened, you will receive an invitation to join.

invitation to join breakout room

If you accept the invitation, you will be moved to a breakout room. From within the breakout room, you will not be able to interact with others in the main Zoom room. For this reason, you will need to use Slack to request help if you need it when you are within a breakout room.

joining a breakout room later

As long as the breakout rooms are open, you will have the option to enter your breakout room.

Screen Sharing

Within your breakout room, you may be asked to share your screen so than an instructor can help you troubleshoot any problem you may be encountering. To do so, simply click on “share screen” in the controls menu at the bottom of the Zoom window.

Remote Control

In some cases, an instructor may request control of your computer once you have shared your screen. You will see a pop-up window.

remote control request

If you approve, the person you are sharing your screen with will be able to move your cursor and type to any open application. You can stop remote control at any time.

remote control options