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      Single Cell RNA-Seq Analysis

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Prerequisites
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Data Reduction
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Project setup
Generating Expression Matrix
scRNAseq Analysis
Prepare scRNAseq Analysis
Part 1- Create object
Part 2- Filtering
Part 3- Normalization and scaling
Part 4- Dimensionality reduction
Part 5- Clustering and cell type
Part 6- Enrichment and DE
Part 7- Doublet detection
Part 8- Integration

Schedule

All posted times are Pacific time zone. At least one break will be provided each morning and afternoon.

Monday, December 11

8:50 am Zoom session starts
9:00 am Introductions
9:30 am Single Cell library prep talk by Dr. Hong Qiu from the DNATech Core
10:30 am Introduction to the command line
12:00 am Lunch
1:00 pm Project set-up
2:00 pm Creating an expression matrix
5:00 pm Zoom session ends

Tuesday, December 12

8:50 am Zoom session starts
9:00 am Introduction to R
11:00 am Installing R packages
11:30 am Parse BioSciences Sponsor Talk
12:00 pm Lunch
1:00 pm Data Analysis Parts 1 - 3
5:00 pm Zoom session ends

Wednesday, December 13

8:50 am Zoom session starts
9:00 am Data Analysis Parts 4 - 5
11:30 am 10X Genomics Sponsor Talk
12:00 pm Lunch
1:00 pm Data Analysis Parts 6 - 8
5:00 pm Zoom session ends