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      Introduction to Single Cell RNA-Seq Workshop

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Introduction and Lectures
Intro to the Workshop and Core
Schedule
What is Bioinformatics/Genomics?
Experimental Design and Cost Estimation
Single Cell Sample Preparation - Dr. Diana Burkart-Waco
Biology at True Resolution - Introduction to Single Cell and Visium Spatial Solutions
Visium Spatial Protocols - Tissue Preparation Guide
Support
Using Slack in this workshop
Using Zoom in this workshop
Cheat Sheets
Software and Links
Scripts
Prerequisites
CLI - Logging in and Transferring Files
CLI - Intro to Command-Line
CLI - Advanced Command-Line (extra)
CLI - Running jobs on the Cluster and using modules
R - Getting Started
R - Intro to R
R - Prepare Data in R (extra)
R - Data in R (extra)
More Materials (extra)
Data Reduction
Project setup
Generating Expression Matrix
scRNAseq Analysis
Prepare scRNAseq Analysis
scRNAseq Analysis - PART1
scRNAseq Analysis - PART2
scRNAseq Analysis - PART3
scRNAseq Analysis - PART4
scRNAseq Analysis - PART5
scRNAseq Analysis - PART6
Shiny App (extra)
Shiny App on AWS (extra)
ETC
Closing thoughts
Workshop Photos
Github page
Biocore website

Schedule

All posted times are Pacific time zone. At least one break will be provided each morning and afternoon.

Wednesday, August 12

8:50 am Zoom session starts
9:00 am Welcome, format and expectations
9:30 am Logging in to the cluster, environment, introductions
10:00 am Introduction to the command line
11:30 am Running jobs on a cluster
12:00 pm Lunch
1:00 pm Genomics/Bioinformatics Talk
3:00 pm Data reduction
4:45 pm Recap and homework
5:00 pm Zoom session ends

Thursday, August 13

8:50 am Zoom session starts
9:00 am Recap
11:00 am Cell Preparation Dr. Diana Burkart-Waco, UC Davis DNA Technologies Core
11:30 am Biology at True Resolution: Introduction to Single Cell and Visium Spatial Solutions 10x Genomics
12:00 pm Lunch
1:00 pm Introduction to R and RStudio
3:00 pm Single cell RNA-Seq analysis in Seurat
4:45 pm Recap and homework
5:00 pm Zoom session ends

Friday, August 14

8:50 am Zoom session starts
9:00 am Shiny App for Single Cell RNA-Seq analysis Keith Mitchell, UC Davis Bioinformatics Core
10:30 am Seurat Analysis, continued
12:00 pm Lunch
1:00 pm Seurat analysis, continued
4:30 pm Closing
5:00 pm Zoom session ends