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      PacBio Iso-Seq Workshop Online

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Introduction
Intro to the Workshop
Schedule
Support
Using Slack in this workshop
Using Zoom in this workshop
Cheat Sheets
Software and Links
Talks
Perspective on Bioinformatics/Genomics?
Iso-Seq Library Prep
Prerequisites
Logging In
CLI
Cluster Computing
Data Reduction
PacBio data
Dr. Liz Tseng
Introduction to PacBio and Iso-Seq
Using the Iso-Seq Application on SMRT Link and BioConda
Tutorial
Richard Kuo
Transcriptome Annotation by Modular Algorithms TAMA
Tutorial
Fairlie Reese
Long-read RNA-seq transcriptomic analysis with TALON and Swan
Tutorial
Download ipython notebook
Conesa Lab
IsoAnnot and IsoAnnotLite
TappAS Install
Hands-on tappAS
ETC
Closing thoughts
Recordings
Github page
Biocore website
Pacific Biosciences website
Conesa lab website

Schedule

All posted times are Pacific time zone. At least one break will be provided each morning and afternoon.

Monday, August 30

8:50 am Zoom session starts
9:00 am Welcome, format and expectations, introductions
9:30 am PacBio Iso-Seq method and Library Prep Kristin Mars, PacBio
10:30 am Introduction to the command line Bioinformatics Core
12:00 pm Lunch
1:00 am Introduction to the command line Bioinformatics Core
2:30 pm Cluster computing and modules Bioinformatics Core
3:00 pm Break
3:15 pm Examining PacBio data (lecture and hands on) Bioinformatics Core
5:00 pm Zoom session ends

Tuesday, August 31

8:50 am Zoom session starts
9:00 am Official PacBio software SMRT Analysis / BioConda (lecture) Dr. Liz Tseng, PacBio
9:30 am Iso-Seq pipeline with Bioconda and visualization using UCSC and IGV (hands on) Dr. Liz Tseng, PacBio
10:30 am Break
11:00 am Cupcake (hands on) Dr. Liz Tseng, PacBio
12:00 pm Lunch
1:00 pm Special Iso-Seq topic (TBA) Dr. Liz Tseng, PacBio
2:45 pm Break
3:00 pm TAMA Richard Kuo, Roslin Institute
5:00 pm Zoom session ends

Wednesday, September 1

8:50 am Zoom session starts
9:00 am TALON & SWAN (demo and hands on) Fairlie Reese, UCI
10:30 am Break
11:00 am SQANTI Dr. Liz Tseng, PacBio
12:00 pm Lunch
1:00 pm IsoAnnot and IsoAnnotLite Bioinformatics Core
2:00 pm tappAS Bioinformatics Core
2:45 pm Break
3:00 pm tappAS Bioinformatics Core
4:30 pm Closing thoughts Bioinformatics Core
5:00 pm Zoom session ends