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      PacBio Iso-Seq Workshop Online

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Introduction
Intro to the Workshop
Schedule
Support
Using Slack in this workshop
Using Zoom in this workshop
Cheat Sheets
Software and Links
Talks
Perspective on Bioinformatics/Genomics?
Iso-Seq Library Prep
Prerequisites
Logging In
CLI
Cluster Computing
Data Reduction
PacBio data
Dr. Liz Tseng
Introduction to PacBio and Iso-Seq
Using the Iso-Seq Application on SMRT Link and BioConda
Tutorial
Richard Kuo
Transcriptome Annotation by Modular Algorithms TAMA
Tutorial
Fairlie Reese
Long-read RNA-seq transcriptomic analysis with TALON and Swan
Tutorial
Download ipython notebook
Conesa Lab
IsoAnnot and IsoAnnotLite
TappAS Install
Hands-on tappAS
ETC
Closing thoughts
Recordings
Github page
Biocore website
Pacific Biosciences website
Conesa lab website

tappAS Installation

tappAS ia a Java GUI application that can:

  1. Analyze diversity of isoform annotations.
  2. Calculate differential isoform usage.
  3. Find pathways and functional domains affected by splicing.
  4. Evaluate differences at UTRs.

TappAS

The softare is available on the TappAS website

The github repository is here

Installing tappAS on your computer

tappAS is a Java application and requires few prerequisites.

Finally,

Additional Tutorials for tappAS can be found here