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      PacBio Iso-Seq Workshop Online

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Introduction
Intro to the Workshop
Schedule
Support
Using Slack in this workshop
Using Zoom in this workshop
Cheat Sheets
Software and Links
Talks
Perspective on Bioinformatics/Genomics?
Iso-Seq Library Prep
Prerequisites
Logging In
CLI
Cluster Computing
Data Reduction
PacBio data
Dr. Liz Tseng
Introduction to PacBio and Iso-Seq
Using the Iso-Seq Application on SMRT Link and BioConda
Tutorial
Richard Kuo
Transcriptome Annotation by Modular Algorithms TAMA
Tutorial
Fairlie Reese
Long-read RNA-seq transcriptomic analysis with TALON and Swan
Tutorial
Download ipython notebook
Conesa Lab
IsoAnnot and IsoAnnotLite
TappAS Install
Hands-on tappAS
ETC
Closing thoughts
Recordings
Github page
Biocore website
Pacific Biosciences website
Conesa lab website

Online Workshops with Zoom

Course lecture, discussions, and one-on-one help/troubleshooting will be conducted using a zoom meeting.

Because video is involved, we ask everyone to be respectful and we reserve the right to remove someone if they are being disrespectful or disruptive.

Basic Controls

conrtol menu

The basic controls can be found at the bottom of the Zoom window. These controls allow participants to control their audio and video. You will automatically be muted on joining the call, but can unmute yourself at any time. When the “Participants” icon in the control menu is selected, a panel will expand from the right side of the Zoom window to display a list of participants.

participants panel

Non-verbal Responses

During lectures, our instructors will occasionally request non-verbal feedback from all participants to quickly assess how well the group is following the material, gauge interest in a topic, and keep track of the number of participants that have completed a hands-on exercise. These options can be found at the bottom of the participants panel.

nonberbal response options

Asking Questions

In addition to non-verbal feedback, we want to hear from participants during lecture sections. Please raise your hand using the “raise hand” option beneath the participants panel to get the speaker’s attention. The instructor will invite you to ask your question, and you should unmute yourself and speak. When your question has been answered, lower your hand.

Breakout rooms

During hands-on sections of the course, breakout rooms may be opened to facilitate group work and allow many participants to receive one-on-one assistance at the same time. When breakout rooms are opened, you will receive an invitation to join.

invitation to join breakout room

If you accept the invitation, you will be moved to a breakout room. From within the breakout room, you will not be able to interact with others in the main Zoom room. For this reason, you will need to use Slack to request help if you need it when you are within a breakout room.

joining a breakout room later

As long as the breakout rooms are open, you will have the option to enter your breakout room.

Screen Sharing

Within your breakout room, you may be asked to share your screen so that an instructor can help you troubleshoot any problem you may be encountering. To do so, simply click on “share screen” in the controls menu at the bottom of the Zoom window.

Remote Control

In some cases, an instructor may request control of your computer once you have shared your screen. You will see a pop-up window.

remote control request

If you approve, the person you are sharing your screen with will be able to move your cursor and type to any open application. You can stop remote control at any time.

remote control options