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      PacBio Iso-Seq Workshop Online

Home
Introduction
Intro to the Workshop
Schedule
Support
Using Slack in this workshop
Using Zoom in this workshop
Cheat Sheets
Software and Links
Talks
Perspective on Bioinformatics/Genomics?
Iso-Seq Library Prep
Command Line Interface
Logging In and Transferring Files
Intro CLI
Advanced CLI
Advanced CLI Challenge Solutions
Clusters and Modules
Data Reduction
PacBio data
Dr. Liz Tseng
SMRT Sequencing - How it Works
Using the Iso-Seq Application on SMRT Link and BioConda
IsoPhase - Isoform-Level Phasing
Single-Cell RNA Sequencing using the Iso-Seq Method
Tutorial
Richard Kuo
Transcriptome Annotation by Modular Algorithms TAMA
TAMA (hands-on)
Tutorial
Conesa Lab
Installing tappAS
Quality Control and Characterization of LR Transcriptomes
SQANTI3 hands on and IsoAnnot
Hands-on tappAS
ETC
Closing thoughts
Workshop Photos
Github page
Biocore website
Pacific Biosciences website
Conesa lab website

Logging In & Transferring Files

You should already have an account on our systems

For Macs/Linux - Logging In

  1. Open a Terminal (usually under Applications/Utilities on a Mac), or install iterm2
  2. Cut and paste this into the terminal:

     ssh username@tadpole.genomecenter.ucdavis.edu
    

    where ‘username’ is replaced with your username. Press Enter.

  3. Type in your password. No characters will display when you are typing. Press Enter.

For Macs/Linux - Transferring files

  1. Use scp (secure copy, a remote file copying program):

     scp username@tadpole.genomecenter.ucdavis.edu:full/path/to/file .
    
  2. Replace ‘username’ with your username and replace ‘full/path/to/file’ with the full path to the file you want to transfer. Note that there is a “.” at the end of the command, which is where to put the file, i.e. your current directory. You will have to type in your password.

For Windows - Logging In

Windows needs a terminal to connect with, you have a few options:

  1. Shell for Windows 10
  2. Enable SSH OR
  3. MobaXterm
  4. PuTTY

In putty, mobaxterm:

  1. Open up terminal.
  2. In the Host Name field, type tadpole.genomecenter.ucdavis.edu
  3. Make sure the Connection Type is SSH.
  4. Press “Open”. It will ask you for your username and password.

    In Shell with SSH enabled:

     ssh username@tadpole.genomecenter.ucdavis.edu
    

    where ‘username’ is replaced with your username. Press Enter.

For Windows - Transferring files

  1. Open up WinSCP. If you haven’t installed it, get WinSCP here.
  2. In the Host Name field, type tadpole.genomecenter.ucdavis.edu
  3. Type in your username and password.
  4. Make sure the File Protocol is SFTP.
  5. Press “Login”.