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      PacBio Iso-Seq Workshop Online

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Introduction
Intro to the Workshop
Schedule
Support
Using Slack in this workshop
Using Zoom in this workshop
Cheat Sheets
Software and Links
Talks
Perspective on Bioinformatics/Genomics?
Iso-Seq Library Prep
Command Line Interface
Logging In and Transferring Files
Intro CLI
Advanced CLI
Advanced CLI Challenge Solutions
Clusters and Modules
Data Reduction
PacBio data
Dr. Liz Tseng
SMRT Sequencing - How it Works
Using the Iso-Seq Application on SMRT Link and BioConda
IsoPhase - Isoform-Level Phasing
Single-Cell RNA Sequencing using the Iso-Seq Method
Tutorial
Richard Kuo
Transcriptome Annotation by Modular Algorithms TAMA
TAMA (hands-on)
Tutorial
Conesa Lab
Installing tappAS
Quality Control and Characterization of LR Transcriptomes
SQANTI3 hands on and IsoAnnot
Hands-on tappAS
ETC
Closing thoughts
Workshop Photos
Github page
Biocore website
Pacific Biosciences website
Conesa lab website

Online Workshops with Slack

Text based communication will be conducted via a Slack channel. Staff will be monitoring the Slack channel to answer questions (and schedule a Zoom break out room if needed). If you know the answer to someone else’s question, feel free to answer it.

Mentions

If you want to make sure that a particular person sees your comment in Slack, mention them by name, preceded by an “@” symbol.

mentioning another user

Threads

We like to use threads to keep messages on a single subject together. To reply to a comment in a thread, hold your mouse over the comment, and select the option “respond in thread.”

starting a thread

In the Slack desktop client, this opens a new window to the right of the main channel.

replying to a thread

Pinned Posts

Pinning a post can keep it from getting lost when the channel gets busy. To pin a post, hold your mouse over the post, select “more,” and choose “pin.”

pinning a post

Pinned posts in a channel can be found quickly by clicking the channel information icon and selecting “Pinned” in the sidebar that appears.

finding pinned posts

Searching Slack

Slack history is searchable. You can specify which channels to search, which people sent a message, and message content.

searching slack