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      Advanced Single Cell RNA-Seq Workshop

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Introduction and Lectures
Intro to the Workshop and Core
Schedule
What is Bioinformatics/Genomics?
Experimental Design and Cost Estimation
Single Cell Sample Preparation - Dr. Diana Burkart-Waco
Support
Cheat Sheets
Software and Links
Scripts
Prerequisites
CLI - Logging in and Transferring Files
CLI - Intro to Command-Line
CLI - Advanced Command-Line (extra)
CLI - Running jobs on the Cluster and using modules
R - Getting Started
R - Intro to R
R - Prepare Data in R (extra)
R - Data in R (extra)
More Materials (extra)
Data Reduction
Generating Expression Matrices
Expression project setup
Preprocessing reads with HTStream
Generating Expression Tables
VDJ T cell and B cell
Velocity analysis
Data analysis
scRNA analysis prepare
Mapping Comparison
Anchoring (Comparison dataset)
Shiny App Install/Overview
App Practical Usage
AWS Hosted App (Optional)
Monocle
VDJ T cell and B cell analysis
Velocity analysis
ETC
Closing thoughts
Workshop Photos
Github page
Biocore website

Tentative Schedule

Day 1

8:50 am Zoom session starts
9:00 am Welcome, format and expectations and introductions
9:30 am Connections, logging in, environment
9:40 pm Intro/Adv to Command Line (Self)
9:45 pm Hands on
10:15 am 15 minute break
10:30 am Running jobs on a cluster
11:00 pm Hands on
11:15 am Genomics/Bioinformatics Talk
12:00 pm Lunch hour
1:00 pm Expression Matrices
1:15 pm Expression Project Setup
1:30 pm Hangs_on
1:45 pm HTStream Preprocessing
2:00 pm Hands_on
2:30 pm 15 minute break
2:45 pm Creating References
3:00 pm Hands_on
3:15 pm Read counting with Cellranger/Star solo/Salmon alevin
3:45 pm Hands_on
4:45 pm Recap and Homework
5:00 pm Zoom session ends

Day 2

8:50 am Zoom session starts
9:00 am Recap and check everyones session
9:15 am Go over mapping indexes
9:45 am Go over mapping scripts
10:15 am 15 minute break
10:30 am Review mapping output
11:00 am Experimental Design Talk
11:45 am Review R expectations
12:00 pm Lunch hour
1:00 pm Mapping Comparison in R
1:30 pm Hands on
2:00 pm Anchoring in R
2:30 pm Hands on
3:00 pm 15 minute break
3:15 pm Shiny App by Keith Mitchell
* Shiny Practical
* Shiny in AWS
4:45 pm Recap and Homework
5:00 pm Zoom session ends

5:00pm - 6:00pm Happy hour discussion

Day 3

8:50 am Zoom session starts
9:00 am Recap and questions
9:30 am Monocle (by Hannah)
10:00 am Hands on 10:45 am 15 minute break
11:00 am Cell Preparation Talk by Diana Burkard-Waco
12:00 pm Lunch hour
1:00 pm VDJ data reduction
1:30 pm Hands on
2:00 pm VDJ anaysis
2:15 pm Hands on
2:30 pm VDJ anaysis
2:45 pm Hands on
3:00 pm 15 minute break
3:15 pm Volocity data reduction 3:30 pm Hands on
3:45 pm Velocity data analysis
4:00 pm Hands on
4:45 pm Closing
5:00 pm Zoom session ends