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      Advanced Single Cell RNA-Seq Workshop

Home
Introduction and Lectures
Intro to the Workshop and Core
Schedule
What is Bioinformatics/Genomics?
Experimental Design and Cost Estimation
Single Cell Sample Preparation - Dr. Diana Burkart-Waco
Support
Using Slack in this workshop
Using Zoom in this workshop
Cheat Sheets
Software and Links
Scripts
Prerequisites
CLI - Logging in and Transferring Files
CLI - Intro to Command-Line
CLI - Advanced Command-Line (extra)
CLI - Running jobs on the Cluster and using modules
R - Getting Started
R - Intro to R
R - Prepare Data in R (extra)
R - Data in R (extra)
More Materials (extra)
Data Reduction
Generating Expression Matrices
Expression project setup
Preprocessing reads with HTStream
Generating Expression Tables
VDJ T cell and B cell
Velocity analysis
Data analysis
scRNA analysis prepare, part 1
Mapping Comparison
Anchoring (Comparison dataset)
Shiny App Install/Overview
Shiny App Practical Usage
AWS Hosted App (Optional)
scRNA analysis prepare, part 2
Monocle
VDJ T cell and B cell analysis
Velocity analysis
ETC
Closing thoughts
Workshop Photos
Github page
Biocore website

Logging In & Transferring Files

You should already have an account on our systems

For Macs/Linux - Logging In

  1. Open a Terminal (usually under Applications/Utilities on a Mac), or install iterm2
  2. Cut and paste this into the terminal:

     ssh username@tadpole.genomecenter.ucdavis.edu
    

    where ‘username’ is replaced with your username. Press Enter.

  3. Type in your password. No characters will display when you are typing. Press Enter.

For Macs/Linux - Transferring files

  1. Use scp (secure copy, a remote file copying program):

     scp username@tadpole.genomecenter.ucdavis.edu:full/path/to/file .
    
  2. Replace ‘username’ with your username and replace ‘full/path/to/file’ with the full path to the file you want to transfer. Note that there is a “.” at the end of the command, which is where to put the file, i.e. your current directory. You will have to type in your password.

For Windows - Logging In

Windows needs a terminal to connect with, you have a few options:

  1. Shell for Windows 10
  2. Enable SSH OR
  3. MobaXterm
  4. PuTTY

In putty, mobaxterm:

  1. Open up terminal.
  2. In the Host Name field, type tadpole.genomecenter.ucdavis.edu
  3. Make sure the Connection Type is SSH.
  4. Press “Open”. It will ask you for your username and password.

    In Shell with SSH enabled:

     ssh username@tadpole.genomecenter.ucdavis.edu
    

    where ‘username’ is replaced with your username. Press Enter.

For Windows - Transferring files

  1. Open up WinSCP. If you haven’t installed it, get WinSCP here.
  2. In the Host Name field, type tadpole.genomecenter.ucdavis.edu
  3. Type in your username and password.
  4. Make sure the File Protocol is SFTP.
  5. Press “Login”.